SUP-18 is expressed predominantly in muscles and co-localizes subcellularly with SUP-10.

<p>Epifluorescence images of worms carrying (A–C) a P<i>sup-10::gfp</i> translational fusion transgene or (D–F) a P<i>sup-18::gfp</i> translational fusion transgene. (A, D) Body-wall muscle cells displaying GFP fluorescence in dense body-like structures. (B, E) Tail regions of transgenic animals showing fluorescence in the anal depressor muscles (arrows). (C, F) Ventral views of transgenic animals showing fluorescence in vulval muscles (arrows). (G, H, I) Confocal microscopic images of an animal expressing a P<i>sup-10::gfp</i> translational fusion transgene and a P<i>_myo-3sup-18</i> transgene. SUP-10::GFP fusion protein (G) was visualized by GFP signals and SUP-18 (H) was detected by immunostaining with a rabbit anti-SUP-18 polyclonal antibody (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004175#s4" target="_blank">Materials and Methods</a>). (I) The merged picture indicates colocalization of SUP-10::GFP and SUP-18 in the dense bodies (arrows). Scale bars, 10 µm.