Arf4 mutant mice have functional nodal cilia.

<p>A. SEM of 3–4 somite developmentally matched embryos at 2,500× (top), 10,000× (middle) and 20,000× (bottom) magnification showing nodal cilia in wild type (left column) and Arf4 mutant (right column) embryos. B. Nodal cilia length is not significantly affected by the Arf4 mutation. Mean cilia length from 3–4 somite stage embryos is plotted (N = 3 each genotype); error bars are standard error of the mean. C. Total number of nodal cilia is not significantly affected in the Arf4 mutants. Mean number of cilia per node (N = 3 for each genotype) is plotted; error bars are standard error of the mean. D. SEM of developing heart tube at E9.5 showing normal heart looping in wild type (left) and Arf4 mutant (right) embryos. E. Heart looping analysis of E9.5 embryos shows that all had normally looped hearts (n = 22, 38, 18 for +/+, +/−, −/−).