Restoration of IGF-1 inhibited miR-190b-mediated insulin resistance.

<p>(A) Western blotting of IGF-1 expression in Huh7-Pre-NC-1 cells or Huh7-Pre-miR190b-6 cells transfected with IGF-1 expression vector in different amounts. (B) Western blotting of expression and insulin-stimulated phosphorylation of IRS1, FOXO1 and GSK3β in Huh7-Pre-NC-1 cells transfected with empty vector, and Huh7-Pre-miR190b-6 cells transfected with empty vector or IGF-1 expression vector. β-actin was used as an internal control.