IL-33 expression in skin after <i>Staphylococcus aureus</i> infection.

<p>(<b>A</b>) Western blot of IL-33 in skin extracts from <i>S.aureus</i>-infected patients. Samples from 3 <i>S.aureus</i>-infected patients with abscess were pooled together. (<b>B</b>) Immunofluorescence analysis of IL-33 in skin of <i>S.aureus</i>-infected patients. This image is representative of three patients with abscess. Dotted white lines indicate the location of dermal-epidermal junction. Red scale bar represents 10 µm and white scale bars represent 50 µm. The arrow designates region of 200× magnification shown in inset. (<b>C</b>) Western blot of IL-33 in skin extracts from <i>S.aureus</i>-infected mice. Samples from 3 <i>S.aureus</i>-infected mice were pooled together. (<b>D</b>) Quantification of IL-33 expression in skin and the lesional size of skin lesions after mouse back skin infected with 1–2×10⁷ CFU <i>S.aureus</i>. Normal skin without infection was collected as control. (<b>E</b>) Immunofluorescence analysis of IL-33 and F4/80 (macrophage marker) in mouse skin after <i>S.aureus</i> infection. This image is representative of six mice with abscess. Dotted white lines indicate the location of dermal-epidermal junction. Red scale bar represents 10 µm and white scale bar represents 50 µm. Arrows designate region of 200× magnification shown in insets. (<b>F</b>) IL-33 expression in RAW264.7 cells stimulated with different doses of heat-inactivated <i>S.aureus</i> for 24 hours. <i>*P</i><0.05 and <i>***P</i><0.001. <i>P</i> values were analyzed by one-way analysis of variance (ANOVA). Data are the means ± SEM and representative of two to three independent experiments with <i>n</i> = 3–6 per group.