IL-33 induces iNOS in macrophages.

<p>(<b>A</b>–<b>C</b>) iNOS expression induced by heat-inactivated <i>S.aureus</i> (<b>A</b>) or PGN (<b>B</b>) or LTA(<b>C</b>) before or after IL-33 was silenced in primary peritoneal macrophages. (<b>D</b>) Quantification of iNOS mRNA expression of WT and <i>Tlr2^−/−</i> primary peritoneal macrophages treated with 10 µg/ml PGN. (<b>E&F</b>) iNOS mRNA expression in BMDMs treated with 30 ng/ml full-length IL-33(<b>E</b>) or in primary peritoneal macrophages treated with 30 ng/ml processed IL-33(Ser109-Ile266) (<b>F</b>) for various times. (<b>G</b>&<b>H</b>) Western blot of iNOS in BMDMs treated with 30 ng/ml full-length IL-33(<b>G</b>) or in primary peritoneal macrophages treated with 30 ng/ml processed IL-33(Ser109-Ile266) (<b>H</b>) for various times. <i>*P<0.05</i>, <i>**P<0.01</i> and <i>***P<0.001</i>. <i>P</i> values were determined by one-way ANOVA (<b>A–C, E&F</b>) or two-way ANOVA (<b>D</b>). All data are means ± SEM of <i>n</i> = 3 and representative of two to three independent experiments.