Mouse NSCs and differentiated NSCs in culture.

<p><b>a</b>) NSC appeared as “Neurospheres” after 10–14 days of culture with NSC medium supplemented with rhEGF and rhFGF growth factors (10X magnification). <b>b)</b> The differentiation of NSCs was induced by incubating the cells in the NSC medium with differentiation supplements. The early stages of the differentiation, where mixed cells are seen emerging from the neurospheres (10X magnification). Scale bars = 100 µm.