Effect of LOV treatment before viral inoculation (pre-treatment).

<p>Groups of six mice were treated with LOV (200 mg/kg/day) orally prior to intraperitoneal inoculation with 1×10⁶ PFU/ml of virus. One group was treated with a single dose (24 hrs before viral inoculation), and the other group was treated with three doses (72, 48 and 24 hrs before viral inoculation). Three types of controls groups were used. The “PBS” group consisted of mice that were not treated with LOV and were not infected, the “CONTROL” group consisted of mice that were treated with LOV but were not infected and the “CONTROL WITHOUT LOV” group consisted of mice that were not treated with LOV but were inoculated with DENV-2. The viremia and survival rates were compared between the control without LOV group and the treated groups (single- and three-dose groups). A. Plaque assay of infected VERO cells to quantify serum viremia at 3 dpi. Bars represent the standard error of the mean (SEM). B. Average body weight percentage, recorded every two days until the animals died or were sacrificed. C. Kaplan-Meier curve showing the survival rates of the untreated group and groups treated with one or three doses of LOV. D. Comparison of survival rates between the untreated group and groups treated with one or three doses. Asterisks in A and D indicate statistically significant differences between the treated LOV groups and the control without LOV group. In the group pre-treated with three LOV doses, there was a significant reduction in viremia. Moreover, the survival rates were increased in both groups (pre-treated with one or three doses).