Enhanced production of MIF, MMP-9 and MMP-2 in the MIF Tg mouse skin following chronic UVB exposure.

<p>(A) Western blot analysis of MIF, MMP-2 and MMP-9 was conducted to evaluate the effects of UVB (300 mJ/cm²×3/week) on the expressions of these compounds in comparison with those observed in WT mice. The band of β-actin was used to normalize each expression level. Each band shows a representative result of the Western blotting analyses, which were repeated five times. A densitometric analysis was also performed to evaluate the intensity of each band. (B) Zymography, using the total skin specimens of each indicative sample, is shown. (These experiments were repeated two times with similar results).