The dynamic curve of adhesion measurements for different kinds of cells: (A–B) CL1-0 (C–D) MRC-5 (E–F) Hs68.

<p>The shear stresses are 3, 7.5, 10, 15, and 30 dyne/cm² applied in all three cell lines. The control experiment is the cell cultured in the microchannel without the medium flowing. (A, C, E) The spectra change under 15 dyne/cm² shear stress stimulation. The black lines show the initial spectrum (I). The red lines show the maximum changes of spectrum (II). The blue lines show the final spectrum after stopping the flow (III). Note, redshift of spectrum in CL1-0 but blueshift in MRC-5 and Hs68. (B, D, F) The amounts of adhesion changes in dynamically for CL1-0, MRC-5, and Hs68 cells under shear stresses of 3, 7.5, 10, 15, and 30 dyne/cm², respectively. The shear flow starts at 5 min and stops at about 25 min.