SLO-perforation inflicts mechanical damage and triggers lysosomal fusion.

<p>(<b>A</b>) HEK 293 cells do not adhere extensively to the substratum at the cellular periphery while SH-SY5Y cells are firmly attached by multiple protrusions. Magnification bars = 2 µm. (<b>B</b>) Inhibition of myosin contraction does not protect from SLO induced lysis. (<b>C</b>) Lysosomal exocytosis (β-hexosaminidase release) after SLO-injury is more pronounced in SH-SY5Y cells compared to HEK 293 cells. (<b>D</b>) Vacuolin-1 does not increase the SLO-induced lysis in HEK 293 cells. In contrast, Vacuolin-1-treated SH-SY5Y cells are more prone to the SLO-induced lysis. *p<0.01.