IL-23R+ cells in the intestine are mostly innate lymphoid cells.

<p>(A) The percentage and the absolute number of IL-23R-eGFP+ cells in lymphoid organs is depicted. The horizontal bar represents the mean of each group. Each symbol represents data from one mouse. (B) The extracellular staining strategy for the identification of specific immune cellular subsets from total cells in the lamina propria of the small intestine and colon is shown. Electronic gating of viable hematopoietic cells was achieved by using the viable exclusion dye and CD45 expression. γδ T cells (CD3ε+ TCR γδ+), NK cells (CD49b+), B cells (CD19+) and innate lymphoid cells (CD117+CD90+). (C) Identification of IL-23R-eGFP+ immune cell subsets from IL-23R-eGFP^+/− mice based on the strategy defined in B. (D) Flow cytometry profiles of IL-23R-eGFP expression in CD11c and CD11b expressing cells. n = 2.