PIAS1 regulates DNA methylation status of the target genes.

<p>(<b>a</b>) DNA Methylation analyses of the indicated loci were performed by bisulfite conversion of genomic DNA from MDA-MB231 cells containing control shRNA or PIAS1 shRNA2. The <b><i>x</i></b> axis represents the positions of the CpG sites relative to the transcription start site (+1); the <b><i>y</i></b> axis represents the percentage. (<b>b</b>) Chromatin immunoprecipitation (ChIP) assay. Extracts from MDA-MB231 cells containing control shRNA, or PIAS1 shRNA2 were immunoprecipitated with anti-DNMT1, anti-DNMT3A or IgG. The bound DNA was quantified by Q-PCR with <i>CCND2</i> promoter-specific primers and presented as “percent of input” (% input). Shown in each panel is a representative of 3 independent experiments. Error bars represent SD.