PIAS regulates the histone marks of the target genes.

<p>(<b>a</b>) Chromatin immunoprecipitation (ChIP) assay. Extracts from MDA-MB231 cells containing control shRNA, or PIAS1 shRNA2 were immunoprecipitated with anti-PIAS1 or IgG. The bound DNA was quantified by Q-PCR with gene-specific primers and presented as “percent of input” (% input). (<b>b</b>) Same as in <b>a</b> except that antibodies specific for acetylated histone H3 (AcH3), histone H3 trimethylated at Lys9 (H3K9me3), or histone H3 trimethylated at Lys27 (H3K27me3) were used, and the levels of these histone marks at the <i>WNT5A</i> loci were quantified. (<b>c</b>) Same as in <b>b</b> except that histone marks at the <i>CCND2</i> promoter were quantified. (<b>d</b>) Same as in <b>b</b> except that the level of H3K9me3 at the heterochromatin region <i>Satellite 2</i> was quantified. (<b>e</b>) Same as in <b>b</b> except that histone marks at the <i>CCND1</i> promoter were quantified. Shown in each panel is a representative of 3 independent experiments. Error bars represent SD.