<p>*Calculated from the exponential decay phase in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0089374#pone-0089374-g004" target="_blank"><b>Figure 4A</b></a> of main paper. ND: not determined. Computed inactivation rates for the exponential denaturation rates for IAP and ChimAP were similar (see text).</p
Traditionally, the dependence of enzyme activity on temperature has been described by a model consis...
<p>Thermal stability of the PGK1 wild-type and variants at 45°C (panel A) and at 37°C (panel B). Eac...
Heat induced enzyme inactivation or protein denaturation is now well documented, due to progresses i...
The denatured states of proteins have always attracted our attention due to the fact that the denatu...
<div><p>The denatured states of proteins have always attracted our attention due to the fact that th...
<p>Red, wild-type bglTm; blue, T1 mutant; green, T2 mutant. The stability of the wild-type bglTm pro...
<p>The kinetics of heat inactivation of the extracellular proteinase from <em>Pseudomona...
When kept in aqueous solution at ambient temperature many proteins undergo denaturation over a rela...
a<p>determined at 3°C/min scan rate.</p>b<p>mean±s.d. from independent experiments performed at thre...
The denatured states of proteins have always attracted our attention due to the fact that the denatu...
The kinetics of heat inactivation of the extracellular proteinase from Pseudomonas fluorescens 22F w...
<p>(A) Concentration-dependence of HBCK inactivation by GdnHCl. The other four enzymes had similar i...
<p>(A) Kinetics of thermal inactivation experiments probing dynamics of heat-induced loss of functio...
<p>(A) The relative enzyme activity was assayed by d-tagatose formation for 30 min. Activity at the ...
Isothermal guandine hydrochloride (GdnHCL)-included denaturation curves obtained at 14 different tem...
Traditionally, the dependence of enzyme activity on temperature has been described by a model consis...
<p>Thermal stability of the PGK1 wild-type and variants at 45°C (panel A) and at 37°C (panel B). Eac...
Heat induced enzyme inactivation or protein denaturation is now well documented, due to progresses i...
The denatured states of proteins have always attracted our attention due to the fact that the denatu...
<div><p>The denatured states of proteins have always attracted our attention due to the fact that th...
<p>Red, wild-type bglTm; blue, T1 mutant; green, T2 mutant. The stability of the wild-type bglTm pro...
<p>The kinetics of heat inactivation of the extracellular proteinase from <em>Pseudomona...
When kept in aqueous solution at ambient temperature many proteins undergo denaturation over a rela...
a<p>determined at 3°C/min scan rate.</p>b<p>mean±s.d. from independent experiments performed at thre...
The denatured states of proteins have always attracted our attention due to the fact that the denatu...
The kinetics of heat inactivation of the extracellular proteinase from Pseudomonas fluorescens 22F w...
<p>(A) Concentration-dependence of HBCK inactivation by GdnHCl. The other four enzymes had similar i...
<p>(A) Kinetics of thermal inactivation experiments probing dynamics of heat-induced loss of functio...
<p>(A) The relative enzyme activity was assayed by d-tagatose formation for 30 min. Activity at the ...
Isothermal guandine hydrochloride (GdnHCL)-included denaturation curves obtained at 14 different tem...
Traditionally, the dependence of enzyme activity on temperature has been described by a model consis...
<p>Thermal stability of the PGK1 wild-type and variants at 45°C (panel A) and at 37°C (panel B). Eac...
Heat induced enzyme inactivation or protein denaturation is now well documented, due to progresses i...