HTS against the NIH Clinical Collection for HuAoSMC proliferation.

<p>Assays were performed with SMCs using the automated assay system as described in detail in Materials and Methods. DMSO (blue, final 0.05% in each of 8 wells) and resveratrol (red, final 50 µM in each of 8 wells) served as negative control and positive control, respectively, on each of six 96-well plates. Total 447 compounds in the NIH Clinical Collection (yellow) were tested at a final concentration of 5 µM (1 well for each drug). <i>For confirmation of the hits with a different method (Cell Titer Glo), please see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0089349#pone.0089349.s002" target="_blank">Figure S2</a>. </i><b>A</b>). Percent Alamar Blue fluorescence reading. The dashed line marks 50% inhibition of SMC proliferation. <b>B</b>). Consistency of HTS assay on each of six 96-well plates.