Differential inhibition of HuAoSMC versus HuAoEC proliferation by the 11 hits selected from the NIH Clinical Collection.

<p><b>A).</b> HTS of the NIH Clinical Collection was performed using SMCs as well as ECs, as described for <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0089349#pone-0089349-g002" target="_blank">Figure 2</a>. Percent inhibition of SMC proliferation (Black) by the 11 hits was compared with that of EC proliferation (red). The vertical bar highlights greater inhibition of SMC versus EC proliferation by idarubicin, which is opposite to the effect of most of the other hits. <b>B).</b> Inhibition of cell proliferation by rapamycin was compared between HuAoSMCs and HuAoECs. The experiment was performed using the automated assay system as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0089349#pone-0089349-g001" target="_blank">Figure 1</a>. Rapamycin was added to a final concentration of 200 nM. Cell number was assessed by Cell Titer Glo assay. Each bar represents a mean ± SD (*P<0.05).