Binding of TFEB to the HEXA gene promoter <i>in vitro</i> and <i>in vivo</i>.

<p>A, Protein binding analysis by EMSA. Controls were run either without NE or with an additional 100-fold molar excess of unbiotinylated promoter segment as competitor DNA. B, Characterization of protein binding by super-shift analysis. HuDe NE was incubated with the segment −71/−104. The addition of NE, unspecific (anti-USF; 1 µg/assay) or specific antibody (anti-TFEB; 1 µg/assay) is indicated. C, ChIP assay using anti-TFEB or IgG control antibodies was performed on chromatin isolated from HuDe starved cells. An equivalent amount of chromatin was used as ‘input’ DNA. PCR products of the HEXA promoter region (left panel) and HEXA exon 11 control region (right panel) run on a 2% agarose gel are shown.