Confirmation of DNA microarray results with quantitative real-time reverse-transcription PCR (RT-qPCR).

<p>Log₂ fold changes of transcript levels measured with DNA microarrays (x-axis) and RT-qPCR (y-axis) in <i>C. botulinum</i> ATCC 3502 cultures 1 h after temperature downshift from 37°C to 15°C. 16S <i>rrn</i> transcript levels were used as a normalization reference in the RT-qPCR. Linear regression analysis showed an R² correlation value of 0.93 between the microarray and RT-qPCR transcription fold change results.