Macroautophagyc pathway and chaperone-mediated autophagy in control and HD human skin fibroblasts.

<p>(A) LC3 immunocytochemistry (green) and total nuclei stained with bis-benzimide (blue) and the percentage of LC3 positive cells with respect to the total cell number. (B) LAMP2A immunocytochemistry (green) and total nuclei stained with bis-benzimide (blue) and percentage of LAMP2A positive cells. (C) LAMP2A (green) and HSC70 (red) colocalization (yellow) and percentage of LAMP2A and HSC70 colocalization respect to the total cell number. (Scale bar = 20 µm). Values are expressed as the mean ± SD, <i>n</i> = 4 patients. Control cell number (mean per field) 30.94±2.012, <i>n</i> = 4. HD cell number (mean per field) 46.89±4.587, <i>n</i> = 4. The data of each patient was obtained using 4 replicates. Statistical analysis was performed by one-way ANOVA with repeated measures followed by Bonferroni multiple comparison test: **p<0.01, ***p<0.001 <i>vs</i> Solvent; +<0.05, +p<0.05, ++p<0.01, +++p<0.001 HD <i>vs</i> controls; ΔΔΔp<0.001 trehalose + epoxomicin <i>vs</i> epoxomicin.