Effect of microinjection of Spire and Fmn2 protein fragments on the cytoplasmic actin network density of mouse oocytes.

<p>Confocal microscopy images of the F-actin cytoplasmic network (Alexa Fluor-488 phalloidin) in fixed oocytes microinjected with Nt-Spire (A), KIND (C), FSI (E), FH2 (G), or FH1_D-FH2 (I). The bar graphs (B, D, F, H, and J) show the intensity of Alexa Fluor-488 phalloidin measured from a single section in the equatorial region of the oocyte. Data were pooled from three independent experiments for FH1_D-FH2, FH2, and KIND, four independent experiments for FSI, and six independent experiments for Spire protein microinjections. Error bars indicate standard deviation.