Time course of NIRFlabeled anti-Siglec-F distribution in the lung.

<p><i>In vivo</i> lung scans of EAAD, control as well as dexamethasone and beta-escin treated animals before (prescan) and at 6 h, 24 h, 48 h and 72 h after antibody administration. Fluorescence intensity distribution is displayed in normalized counts (NC). In contrast to control mice (A, lower panel, n = 6), OVA-immunized mice have a marked accumulation of anti-SiglecF-750 within the lungs from 24 h, which decreases at 72 h (A, upper panel, n = 8). Anti-SiglecF-680 also reveals significant differences between EAAD (B, upper panel, n = 5) and control (B, lower panel, n = 4) fluorescence intensities derived from the lung. EAAD mice treated with either dexamethasone (C, upper panel, n = 5) or beta-escin (C, lower panel, n = 5) have low intensities over the lung, similar to healthy control mice (A and B, lower panels) at all scan times.