Nuclear translocation of STATc is delayed in pyk3⁻, phg2⁻, and pyk3⁻/phg2⁻ cells.

<p>(A) Ax2 wild-type, pyk3⁻, phg2⁻, and pyk3⁻/phg2⁻ cells expressing GFP-STATc were treated with 100 mM sorbitol for the indicated times to induce GFP-STATc nuclear translocation, fixed with ice-cold methanol, and observed under the fluorescence microscope. Exemplary images of GFP-STATc expressing cells after 0, 3, and 8 min of treatment are shown and the insets at 3 min show a single exemplary cell for each strain to show prominent nuclear (Ax2), transition i.e. beginning nuclear (pyk3⁻ and phg2⁻) and clear cytosolic (pyk3⁻/phg2⁻) localisation of GFP-STATc. (B) Quantification of GFP-STATc nuclear translocation in Ax2 wild-type and mutant cells. For each time point, we analysed 150 cells per experiment and determined the number of cells showing either clear cytosolic (black bar), transition i.e. beginning nuclear (grey bar) or prominent nuclear (white bar) localisation of GFP-STATc. The percentage of cells in each of these three categories was calculated. Error bars depict standard deviations of two independent experiments.