AFOH blocks 3D invasive growth of MDA-MB-231 cells.

<p>Cells were seeded in growth factor reduced Matrigel, treated with DMSO (A, B) or 5 µM AFOH (C, D) for 10 days. Then phase contrast images were taken from randomly chosen fields (A, C). Colonies grown in Matrigel were smeared onto slides, immunostained for F-actin (red) and nuclei (blue), and then imaged by confocal microscopy (B, D). The representative images from three experiments are shown.