NCoA6 knockout MEFs are not permissive for full-length HCV RNA replication.

<p><b>A)</b> NCoA6 knockout MEFs were electroporated with full-length HCV RNA or sub-genomic HCV RNA and the indicated miRNA. Note that sub-genomic HCV RNA uses a firefly luciferase reporter, while full-length HCV RNA uses a Renilla luciferase reporter, and the relative light units measured (RLU) are not directly comparable. <b>B)</b> Supernatant from NCoA6 knockout MEFs electroporated with full-length HCV RNA was harvested five days post-electroporation and plated on Huh7.5 cells, which are permissive for HCV infection and replication without additional modification. Three days post-infection, the Huh7.5 cells were monitored for luciferase expression as an indication of replication. The luciferase levels indicated here are equivalent to background. Results in (A) and (B) are shown with SEM and are an average of three independent experiments.