Assessment of apoptosis imaging <i>in vivo</i> and <i>ex vivo.</i>

<p>Whole-body and excised tissues in bioluminescence images of ES-2-luc-bearing mice and those in fluorescence images of same animals obtained with Xenogen IVIS 200 Series. White-light <i>vs</i>. fluorescence imagings in mid-line incised ES-2-luc-bearing mice obtained with Fluobeam 800. (A) Representative fluorescence and bioluminescence images of ES-2-luc-bearing mouse injected IP with empty PEG-<i>b</i>-PCL micelles followed by an IV injection of methoxy-PEG-<i>b</i>-PCL micelles carrying DiR at 250 µg/kg as a control (left hand side) and those of ES-2-luc-bearing xenograft model injected IP with PEG-<i>b</i>-PCL micelles carrying PTX, CYP, and GSP at 30, 30, and 30 mg/kg followed by an IV injection of PS-targeting PEG-<i>b</i>-PCL micelles carrying DiR at 250 µg/kg as an experimental group (right hand side) at 24 and 48 h after termination of treatments.