Altered dietary protein intake affects plasma and intramuscular AA concentrations and mTORC1 pathway signalling in MS-<i>Slc7a5</i>-KO mice.

<p>Mean ± SEM for n = 6–10 (WT) and 3–5 (MS-<i>Slc7a5</i>-KO) male mice. (A) There were significant effects of dietary protein content (<i>F</i> (2, 27) = 4.97, <i>p</i> = .014) on gastrocnemius leucine concentration. Statistically-significant differences between groups were only detected for MS-<i>Slc7a5</i>-KO animals on different protein diets (**D, p<0.01) as indicated. (B) There were significant effects of both genotype (<i>F</i> (1, 23) = 5.55, <i>p</i> = .027) and dietary protein content (<i>F</i> (2, 23) = 4.49, <i>p</i> = .023) on plasma leucine concentration. Statistically-significant differences between genotype (***G, p<0.001) and dietary protein (***D, p<0.001; wild-type only) groups are indicated. (C) Upper panel shows quantitation of S6K phosphorylation normalised to effect of insulin injection (wild-type mouse on standard chow diet), lower panel shows representative western blot for phospho-S6K and total S6K. Animals injected with NaCl and insulin were used as a negative and positive control, respectively. 2-way ANOVA shows significant effects of both genotype (<i>F</i> (1, 27) = 9.61, <i>p</i> = .004) and dietary protein content (<i>F</i> (2, 27) = 4.97, <i>p</i> = .015) on S6K phosphorylation. Statistically-significant differences between genotype (**G, p<0.01) and diet (**D, p<0.001; wild-type only) groups are indicated.