RAGE regulates infiltration of microglia/macrophages to CNV lesions.

<p>A) The macrophage marker CD68 and isolectin (which binds to microglia and macrophages) were used to assess immune cell infiltration into CNV lesions within the retina of WT and RAGE−/− mice. Representative images demonstrated that Isolectin-positvie cells sometimes co-locaslised with CD68 positive cells. Most isolectin-positive cells demonstrated a dendritic morphology (arrow). (Scale bar = 50 µm). B) Microglia were quantified both in CNV lesions from WT and RAGE−/− mice. Isolectin-positive cells with a typically dendritic (inactive) morphology occurred at high numbers in retinas of non-lasered mice but only in the WT did laser cause a reduction in this phenotype. (** P<0.01). Representative images are shown of typical dendritic and amoeboid microglial cell in the retina. C). Active microglia (with an amoeboid phenotype) were higher in lasered WT and RAGE−/− mice when compared to their non-lesioned controls. RAGE−/− mice had significantly fewer active microglia compared to WT mice (**P<0.01). n = 12 mice/group.