Expression of <i>TLX1</i> in <i>IgHµ-TLX1^TgPrkdc^Scid/Scid</i> premalignant thymocytes increases cell viability and provides a proliferative advantage.

<p>(A) Absolute cell numbers of thymocytes isolated from 20, six week old <i>IgHµ-TLX1^TgPrkdc^Scid/Scid</i> and 20 <i>Prkdc^Scid/Scid</i> littermates (p<0.0001). (B) Thymocytes were flow sorted based on expression of CD44 and CD25 and absolute numbers of DN thymocytes were calculated using percentages obtained after flow sorting (p<0.0001). (C) Thymocytes from three, six week old <i>IgHµ-TLX1^TgPrkdc^Scid/Scid</i> and <i>Prkdc^Scid/Scid</i> littermates were stained with Annexin V and PI than assessed by flow cytometric analysis for cell viability. The lower left quadrant of each panel contains viable cells, the upper right quadrant contains dead cells and the lower right quadrant contains early apoptotic cells. The percentage of cells in each quadrant is indicated. (D) Percentages of viable, apoptotic and dead thymocytes in thymi of <i>IgHµ-TLX1^TgPrkdc^Scid/Scid</i> and <i>Prkdc^Scid/Scid</i> littermates, as determined by flow cytometry with Annexin V and PI staining. Error bars represent SD. (E) Histogram showing premalignant thymocytes obtained from <i>Prkdc^Scid/Scid</i> and <i>IgHµ</i>-<i>TLX1^TgPrkdc^Scid/Scid</i> mice, 2 and 7 hours after intraperitoneal injection with 10 µM BrdU. (F) The percentages of proliferating cells in thymi of <i>IgHµ-TLX1^TgPrkdc^Scid/Scid</i> and <i>Prkdc^Scid/Scid</i> littermates as determined by BrdU and PI staining. Data represent means of triplicate measurements with error bars to represent ± SD (p<0.0001). Statistically significant differences between compared samples are indicated by asterisks.