Validation of differential gene expression in premalignant thymocytes <i>of Prkdc^Scid/Scid</i> and <i>IgHμ-TLX1^TgPrkdc^Scid/Scid</i> mice.

<p>qRT-PCR analysis of selected genes whose protein products are involved in chromosome segregation (<i>Chek1, Aurka</i> and <i>Bub1</i>), cell cycle progression (<i>Cyclin A, Cyclin B1, Anapc5, c-myc</i> and <i>c-myb</i>) and apoptosis (<i>Birc5, Brca1</i>). Samples are presented as pairs with the first bar representing the level of expression of cells isolated from <i>Prkdc^Scid/Scid</i> mice and the second from <i>IgHµ-TLX1</i>^Tg<i>Prkdc^Scid/Scid</i> mice. Data were normalized relative to <i>β-actin</i>. Red bars: DN1; brown: DN2; green: DN3 cells; black; total unsorted thymocytes. Error bars represent SD. Statistical testing was performed using the student’s T-test. Statistically significant differences are indicated by asterisks (* depicts p<0.05, ** depicts p<0.01, *** depicts p<0.001).