Cellularity and localization of labeled RAECs.

<p>(A) Number of DAPI-positive nuclei per mm² of scaffold for each delivery method: the aorta only (Aorta), the BA only (BA), or the combined IVC+BA method (n = 3 hearts per method; mean ±SEM). The total number of cells delivered is indicated in parenthesis. (B, C) Image of whole heart in which 4×10⁷ DiI-labeled RAECs were delivered via the BA. (D, E) Image of whole heart in which 2×10⁷ DiO-labeled cells were delivered via the IVC followed by an additional 2×10⁷ DiI-labeled cells administered via the BA. View of the (F) left ventricular endocardial surface, (G) right ventricular endocardial surface, and (H) the ventricle wall of a heart scaffold recellularized via the BA+IVC cell delivery technique with cells labeled as in D and E. DAPI-positive nuclei are blue (F–H), and overlapping green and red staining shows as yellow (D–H). *p<0.05. Scale bars represent 5 mm (B–E) and 50 microns (F).