Analysis of the NGS libraries.

<p><b>A</b>) Size distribution of sequences remaining after alignment to the <i>Giardia</i> genome and removal of rRNA and tRNA. A peak at 26 nts corresponds to the expected size of miRNAs in <i>Giardia</i>. <b>B</b>) Analysis of read origins in the TDIP1 library. The majority of potential miRNAs are derived from ORFs or unannonated regions. <b>C</b>) Analysis of read origins in the TDIP2 library. <b>D</b>) Analysis of read origins in the TDSIP library shows a 12–13% increase in the reads derived from unannonated regions with a corresponding decrease in reads derived from ORFs.