Infection of Huh7.5_JFH1 cells with HIV results in increased HCV RNA synthesis.

<p>100,000 Huh7.5_JFH1 cells were incubated with DNase-treated HIV_NL4-3 (white bars) or HIV_YK-JRCSF (grey bars) at an MOI = 1 for two hours. At days 1, 3, 7, and 14 post-infection, positive-sense HCV RNA (<b>A</b>) and negative-sense HCV RNA (<b>B</b>) were quantified in cell lysates, compared to the no HIV condition (black bars) by real-time, strand-specific PCR, and normalized to cellular GAPDH levels as described previously <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0083728#pone.0083728-Blackard3" target="_blank">[33]</a>. * p≤0.05; ** p≤0.10 compared to HIV-uninfected Huh7.5_JFH1 cells at each time point.