Preparation of crosslinked starch microspheres and their drug loading and releasing properties

Crosslinked anionic starch microspheres were prepared with sodium trimetaphosphate (STMP) as crosslinking agent through 5 h w/o emulsification-crosslinking reaction at 50 °C. Laser diffraction technique and scanning electron microscopy revealed that microspheres had narrow size distribution, good sphericity and fine dispersibility. In addition, drug loading and releasing properties were investigated with Methylene Blue as a model drug on the basis of single-factor study. It was found that the loading ratio of MB was significantly influenced by loading time, dissolution medium, loading temperature as well as MB concentration (P < 0.05). Either the increase of loading time or drug concentration could lead to the increase of drug loading amount of microspheres, however, drug loading amount reached its maximum in NaCl (0.9%) dissolution medium at room temperature. Furthermore, the release profile contained two main expulsion processes: an initial burst release followed by a sustained swelling-controlled release