Expression of inducible nitric oxide synthase mRNA (<i>NOS2</i>) is increased in the bronchial epithelium of allergic asthmatics.

<p>RNA was extracted from cells isolated from bronchial brush biopsies collected during fiberoptic bronchoscopy of healthy controls (n = 4), and allergic asthmatics (n = 6). The integrity of RNA was assessed, and degraded samples were excluded from the analysis. Gene expression of <i>NOS2</i> (coding for iNOS) relative to glyceraldehyde 3-phosphate dehydrogenase (<i>GAPDH</i>) was analyzed by semi-quantitative real-time PCR. Bars indicate means, errorbars indicate SEM. Significant differences were assessed using unpaired t-test. ** indicates p<0.01.