Relative absolute cell numbers of splenic cell subpopulations in mice hydrodynamically injected with cytokine cDNAs.

<p>C57BL/6 mice were hydrodynamically injected with control, IL-12 and/or IL-18 cDNAs. Up to 50 days post-injection, animals were sacrificed and single cells suspensions of splenic cells were obtained. The percentage of the different subsets was obtained by surface lineage staining with combination of the following Abs: NK1.1, CD3, CD11b, CD19, CD4 and CD8 and analyzed by flow cytometry. Relative cell numbers of the different cell populations were calculated by dividing the absolute cell numbers obtained from the cytokine-treated mice by the absolute cell numbers obtained from control mice. Data is expressed as the mean of a pool of 4 different experiments (total n = 12 mice per group). *12+18 and 12 vs control p<0.05, ^#12 vs 12+18 p<0.05.