Histopathological alterations in hepatic tissue after systemic expression of IL-12 or IL-12+IL-18.

<p>Livers obtained from mice in the different groups were obtained on day 14-16 post-cDNA treatment. Sections of 6 µm were obtained and (<b>A–G</b>) hematoxilin/eosin (H/E) or (<b>H–I</b>) Periodic acid-Schiff (PAS) stains were applied to the sections. (<b>A</b>) Hepatocytes and sinusoids from a control mouse, 100×. (<b>B</b>) Ballooning hepatocytes and absence of hepatic sinusoids in an IL-12-cDNA treated mouse, 100×. (<b>C</b>) Focus of ectopic hematopoiesis in an IL-12-cDNA treated mouse, 100×. (<b>D</b>) Megakaryocyte in an IL-12-cDNA treated mouse, 100×. (<b>E</b>) Mallory and (<b>F</b>) Councilman bodies in an IL-12-cDNA treated mouse, 100×. (<b>G</b>) Focus of necrosis; 10×. Glycogen deposits obtained in a mouse from (<b>H</b>) the control group or (<b>I</b>) the IL-12-cDNA group, 20×. Liver sections from (<b>J</b>) WT or (<b>K</b>) TNFR1 KO mice 15 days after IL-12 cDNA treatment. Pictures are representative of 2 different experiments with 4–5 mice per group.