Expression profiling of KSHV viral genes from ascites cells in NOD/SCID mice.

<p>(<b>A</b>) Ascites fractions were collected and purified as described above, then expression of viral latent gene (<i>Lana, vFlip)</i> and lytic gene (<i>Rta, vGpcr, K8.1)</i> was detected by RT-PCR and agarose gel electrophoresis. <i>β-actin</i> was used as an internal control. (<b>B,C</b>) The expression of viral genes and microRNAs for <i>in vitro</i> cultured BCBL-1 and purified ascites cells from mice (shown as BCBL-1 <i>in vivo</i>) was compared by using qRT-PCR as described in Methods. Error bars represent the S.E.M for three experiments. ** = p<0.01.