HIV-1 colocalizes with CD81-lined vesicle compartments in astrocytes.

<p>(A) SVG astrocyte cells were infected with an EGFP content-labelled HIV-1 YU2 virus (green) and samples were harvested at 135 mins post-infection. Cells were stained with the vesicle/endosomal markers CD81, EEA1, CD63 or CD107b (red) and nuclei counterstained with DAPI (blue). Panels represent the individual fluorescent channels with the overlay (OL) on the far right. Scale bars are 10 μm. Images are representative of multiple fields of view and three independent experiments. (B) Quantification of colocalization of vesicle/endosomal markers with HIV-1 using IMARIS software. Bar graphs and error bars represent the mean and standard deviation, respectively. Data are a compilation of multiple fields of view and three independent experiments.