Luteolin reduces TNF-stimulated mRNA expression of NFKB1 (NF-κB p50) and RELA (NF-κB p65) in keratinocytes.

<p>The effect of luteolin on mRNA expression levels of two genes encoding NF-κB subunits were investigated using qRT-PCR. HaCaT cells were pretreated with luteolin (10–100 µM, 30 min) before stimulation with TNF (50 ng/mL, 6 h): (A) NFKB1, (B) RELA. NHEKs were pretreated with luteolin (10 µM, 30 min) before stimulation with TNF (50 ng/mL, 6 h): (C) NFKB1, (D) RELA. (n = 3, * p<0.05).