Cell cultures containing GBM TICs display higher endogenous ROS generation than normal astrocytes.

<p>Representative experiment of FCM analysis of normal human astrocytes (NA) and cell cultures containing GBM TICs obtained from four different patients (PT1, PT2, PT4, PT5) incubated with the indicated fluorescent probe. Mitotracker Green, TMRE, DCFDA and MitoSOX Red were used to evaluate mitochondrial mass, mitochondrial proton gradient, global ROS and mitochondrial superoxide, respectively.