CMTM3 induces cell cycle arrest and apoptosis of NCCIT cells in a p53-independent manner.

<p>(A) Representative cell cycle analysis. The results are represented as the average ± S.D and are based on three independent experiments (p<0.01). G1 and G2 phases in red, S phases in white. (B) NCCIT cells infected with Ad-null and Ad-<i>CMTM3</i> were labeled with FITC-Annexin V/PI, and apoptosis was assessed by flow cytometry. (C) Western blot analysis of cell cycle- and apoptosis-related proteins in Ad-null- and Ad-<i>CMTM3</i>-infected NCCIT cells. β-actin was used as a control. All experiments were performed three times. (D) A schematic illustration of the proposed model depicting a p53-independent apoptosis pathway in testicular tumors.