High glucose level promotes the migration of MCF-7 breast cancer cells.

<p>A confluent monolayer of MCF-7 cells was scratched and then cultured with normal (5.5 mM), high (25 mM) D-glucose concentration, or an osmotic control (5.5 mM D-glucose plus 19.5 mM D-mannitol or D-fructose) medium as described in the Materials and Methods. After 24 h, the wounded area was observed and photographed by microscopy. <b>(A)</b> Images at 0 and 24 h after scratching of each experimental condition. Arrowed lines represent cell-free zone in wounded area. Bars: 100 µm. <b>(B)</b> Migratory rates of each experimental condition. The quantity was normalized the wound closure area between 0 and 24 h against the wounded area at time zero and expressed as percentage of the migratory rate of MCF-7 cells incubated with the normal glucose concentration. Data are presented as means ± SD. ^***<i>p</i><0.001 versus control (5.5 mM D-glucose concentration), n = 3–7.