Effect of AFP silencing on the expression of MMP-9 and MMP-2, and the invasive ability of Huh-7 cells.

<p>Cells were transfected with control siRNA, vehicle control (LP2000) and AFP-siRNA. The expression of MMP-9 and MMP-2 was examined by Western blot (<b>A</b>) and Real-Time PCR (<b>E</b>). Impact of AFP-siRNA silencing on the invasive ability of Huh-7 cells was examined by chamber invasion assay. Transfection of Huh-7 cells with AFP-siRNA led to a decreased cell invasion (<b>D</b>) compared to cells transfected with control siRNA (<b>B</b>) or LP2000 (<b>C</b>).