Immunohistochemical detection of MeCP2E1 and MeCP2E2 in the adult mouse brain regions.

<p>Left panel <b>(A</b>–<b>J)</b>: MeCP2E1, and right panel <b>(A1</b>–<b>J1)</b>: MeCP2E2. Expression of MeCP2 isoforms was analyzed in seven indicated brain regions of the adult mouse brain. <b>(A</b>–<b>A1)</b> Tiled image of whole hippocampus, and specific regions of hippocampus, <b>(B</b>–<b>B1)</b> CA2, <b>(C</b>–<b>C1)</b> CA3, <b>(D</b>–<b>D1)</b> Dentate gyrus (DG); <b>(E</b>–<b>E1)</b> Olfactory bulb; <b>(F</b>–<b>F1)</b> Striatum; <b>(G</b>–<b>G1)</b> Cortex; <b>(H</b>–<b>H1)</b> Thalamus; <b>(I</b>–<b>I1)</b> Brain stem; <b>(J</b>–<b>J1)</b> Cerebellum. Scale bars represent 200 µm in (A–A1) and 20 µm in (B–J and B1–J1). <b>(K)</b> Detection of MeCP2E1 and MeCP2E2 in three layers of the cerebellum; molecular layer (ml), Purkinje cell layer (pcl) and granule cell layer (gcl). Scale bars represent 20 µm. <b>(L</b>–<b>N)</b> Colocalization of MeCP2E1 and MeCP2E2 in the nuclei in the three layers of the cerebellum; <b>(L)</b> molecular layer, <b>(M)</b> Purkinje cell layer and <b>(N)</b> granular cell layer. Scale bars represent 2 µm. <b>(O)</b> Absence of MeCP2E1-, and MeCP2E2-specific signals in three layers of the cerebellum of the <i>Mecp2</i>^{tm1.1Bird y/−} null mouse brain [molecular layer (ml), Purkinje cell layer (pcl) and granule cell layer (gcl)]. Scale bars represent 20 µm. <b>(P</b>–<b>R)</b> Absence of MeCP2E1/- and MeCP2E2-specific signals in the nuclei in the three layers of the cerebellum of the <i>Mecp2</i>^{tm1.1Bird y/−} null mouse brain; <b>(P)</b> molecular layer, <b>(Q)</b> Purkinje cell layer and <b>(R)</b> granular cell layer. Scale bars represent 2 µm.