Detection of MeCP2E1 and MeCP2E2 in different cell types of the hippocampus (CA1 region) in adult male mouse brain.

<p>Left panel <b>(A</b>–<b>F)</b>: MeCP2E1 and, Right panel <b>(A1</b>–<b>F1)</b>: MeCP2E2. Expression of MeCP2 isoforms was analyzed in neurons (NEUN⁺), astrocytes (GFAP⁺), and oligodendrocytes (CNPase⁺). <b>(A</b>–<b>C)</b> and <b>(A1</b>–<b>C1)</b> represent ×40 images. Scale bars represent 20 µm. <b>(D</b>–<b>F)</b> and <b>(D1</b>–<b>F1)</b> represent confocal images of single nuclei. Scale bars represent 2 µm. <b>(A</b>–<b>A1)</b> and <b>(D</b>–<b>D1)</b>: Expression of MeCP2 isoforms in NEUN⁺ neurons. <b>(B</b>–<b>B1)</b> and <b>(E</b>–<b>E1)</b>: Expression of MeCP2 isoforms in GFAP⁺ astrocytes. <b>(C</b>–<b>C1)</b> and <b>(F</b>–<b>F1)</b>: Expression of MeCP2 isoforms in CNPase⁺ oligodendrocytes. All images were taken from hippocampus of adult male mouse brain.