Expression of the integrin subunits and heterodimers that are targeted by cilengitide.

<p>(A) Relative mRNA levels of cilengitide target integrin subunits were measured by real-time qPCR and normalised to the Glyceraldehyde 3-phosphate dehydrogenase (<i>GAPDH</i>) control gene expression. Each cell line was analysed at least twice with two independent RNA samples. (B) Western blot analysis of αv integrins under non-reducing condition. The β-actin protein serves as loading control. Molecular sizes are indicated on the left of the blots. (C) Immunofluorescence of αvβ3 in MPM cells. The cells were incubated with a rabbit monoclonal antibody against αvβ3 and detected with an Alexa Fluor 488-conjugated secondary antibody.