Parameters included in systematic evaluation of staining conditions for CD31, CD8 and FOXP3 in osteosarcoma samples.

<p>Systematic evaluation of more than 200 different antigen retrieval conditions, different blocking conditions, detection systems and chromogens allowed identification of optimal staining conditions for formalin-fixed and paraffin-embedded osteosarcoma samples.</p><p>* In steps of 3–5°C;</p><p>** in steps of 5 min;</p><p>*** in concentrations of 0.1%–10%, adjusted temperatures and various times and combinations.