Comparison of the recovery of Kv1.3 channels between the experimental data and simulations.

<p>(<b>A–B</b>) The representative currents of recovery from inactivation were obtained in whole-cell patch mode at −90 mV and −45 mV. Two-pulse protocol: from a holding potential of −90 mV, the first pulse 1 (P1) was applied for 1 s at +50 mV, and then the second +50-mV pulse (P2) was applied for 150 ms after a variable interpulse interval at −90 mV in (<b>A</b>) and −45 mV in (<b>B</b>). The detailed recovery currents are shown in the box. The voltage protocol is placed at the bottom. Red lines are fits and black ones are data. The channel numbers for simulations are 3788 for −90 mV and 2740 for −45 mV. (<b>C</b>) Comparison of the time courses of recovery between the experimental data (black) and the simulations (red) at −100 mV, −90 mV, −80 mV and −45 mV. (<b>D</b>) Comparison of time constants of recovery between the experimental data (black) and the simulations (red). The time constants of recovery (mean ± SEM) are 8.6±2.7 s (n = 5) at −45 mV, 15.6±1.6 s (n = 3) at −80 mV, 14.6±2.4 s (n = 7) at −90 mV, 14.6±4.7 s (n = 3) at −100 mV, respectively.