Synaptophysin in the IPL of rd1 mouse was upregulated.

<p>A, B) Representative images of vertical sections of adult wild-type (A) and rd1 (B) mouse retinas immunostained for synaptophysin. Scale bar: 50 µm. C) Representative profile of staining intensity through retinal depth covering both plexiform layers of the images shown in A and B. After background subtraction, a line moving vertically from top to bottom across the images measured the signal intensity using Plot Profile function in ImageJ, which is shown here from left to right. Synaptophysin staining is nearly absent in OPL of rd1 mouse retina, whereas that in IPL is higher than in wild-type, particularly in the Off sublamina. D) Levels of synaptophysin in IPL (mean ± SE), measured with quantitative immunohistochemistry were significantly higher in adult rd1 mouse retina than in wild-type (p = 0.018; n = 6). Within IPL, the levels were significantly higher in Off sublamina (p = 0.01), but not in On sublamina (p = 0.068). The data shown here are essentially the area under the curve for On (60% of IPL) and Off (40%) sublaminas shown in 2C. *<i>p</i><0.05.