Subcellular localization of human FTSJ2 in TE671 and HepG2 cells.

<p>(<b>A</b>) Schematic of the hFTSJ2 over-expression vector. (<b>B</b>) Over-expression of the hFTSJ2 protein in the TE671-h<i>FTSJ2</i> and HepG2-h<i>FTSJ2</i> stable clones. (<b>C</b>) Immunofluorescence staining with anti-hFTSJ2 (green), MitoTracker for mitochondria (red), and DAPI for nuclei (blue) in the TE671-h<i>FTSJ2</i> and HepG2-h<i>FTSJ2</i> cells. (<b>D</b>) Mitochondrial localization of the hFTSJ2 protein in non-transfected TE671 cells. VDAC and MEK-1 were used as the mitochondrial and cytosolic fraction controls, respectively, in the Western blot analysis.